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Four types of polyphenol oxidase were isolated from the crude extract of a Ligularia fischeri by gel filtration on Sephadex G-150. Optimum pH and temperature for the activity of partially purified enzyme were 7.5 and 25℃, respectively. It was stable at temperature 40℃when examined at pH 7.5 for ;i min, and lost 90% of its activity at 70℃ in 30 min at pH 7.5. The enzyme has good activity on catechol and chlorogenic acid but was inactive on dopamine(Received 3, March, 1992, accepted 6, May 1992).
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